Journal: Scientific Reports

Article Title: Methylated (−)-epigallocatechin 3-O-gallate potentiates the effect of split vaccine accompanied with upregulation of Toll-like receptor 5

doi: 10.1038/s41598-021-02346-4

Figure Lengend Snippet: EGCG3”Me intake upregulated TLR5 expression in vivo. ( A ) The chemical structure of EGCG3”Me ( B – E ) 12-week-old female BALB/c mice were orally administrated with vehicle, EGCG (10 mg/kg intragastric administration), or EGCG3”Me (10 mg/kg intragastric administration) every day. After 1 week, mice were sacrifced and TLR5 expression was determined by flow cytometry ( B ) TLR5 expression in lymphocytes, ( C – E ) TLR5 expression in CD11b + F4/80 + cells. (n = 8). ( F ) TLR7 expression was evaluated by flow cytometry (n = 8). Data are presented as means ± SEM * P < 0.05.

Article Snippet: Allophycocyanin (APC)-labeled anti-mouse F4/80 was procured from Miltenyi Biotec (Rhine-Westphalia, Germany), and FITC-labeled anti-mouse CD11c and APC-labeled anti-mouse CD11b, from Miltenyi Biotec.

Techniques: Expressing, In Vivo, Flow Cytometry

Journal: Scientific Reports

Article Title: Methylated (−)-epigallocatechin 3-O-gallate potentiates the effect of split vaccine accompanied with upregulation of Toll-like receptor 5

doi: 10.1038/s41598-021-02346-4

Figure Lengend Snippet: EGCG3”Me intake enhanced TLR5 expression on LPDCs and macrophages of split-virus vaccine-treated mice. ( A–E ) 12-week-old female BALB/c mice were fed AIN-93G diet or AIN-93G diet supplemented with 0.01% EGCG3”Me (EGCG3”Me 20 mg/kg body weight) for 5 weeks. Mice were treated with split-virus vaccine (1 μg HA/mouse i.d.) at 2 and 4 weeks and then sacrificed at 5 weeks. ( A ) CD11c + CD11b + cells ( B, C ) and TLR5 expression on CD11c + CD11b + cells were determined by flow cytometry (n = 6–8). Data are presented as means ± SEM * P < 0.05.

Article Snippet: Allophycocyanin (APC)-labeled anti-mouse F4/80 was procured from Miltenyi Biotec (Rhine-Westphalia, Germany), and FITC-labeled anti-mouse CD11c and APC-labeled anti-mouse CD11b, from Miltenyi Biotec.

Techniques: Expressing, Virus, Flow Cytometry

Journal: MethodsX

Article Title: An in vivo gene delivery approach for the isolation of reasonable numbers of type 2 innate lymphoid cells

doi: 10.1016/j.mex.2020.101054

Figure Lengend Snippet:

Article Snippet: CD11b , APC-Vio770 , Miltenyi Biotec , REA592 , 20.

Techniques:

Journal: bioRxiv

Article Title: Prophylactic TLR9 stimulation reduces brain metastasis through microglia activation

doi: 10.1101/533927

Figure Lengend Snippet: ( a-b ) TAMRA-labeled CpG-C was injected intraperitoneally, 24 hours later brains were perfused, and CpG-C internalization in endothelial cells (CD31), astrocytes (GFAP and GLAST), and microglia (CX3CR1 and CD11b) was visualized in histological sections using confocal microscopy ( a ; top panels are 15μm z-max projections, and lower panels are partial reconstruction); and quantified using ImageStream FACS analysis ( b ). The majority of each of the three cell populations internalized CpG-C, indicating that CpG-C crosses the BBB into the parenchyma (n=4). Scale bar is 5μm. Data presented as mean (±SEM).

Article Snippet: For quantification of CpG-C infiltration into the brain and its internalization by endothelial cells, astrocytes, and microglia , mice were injected with FITC-labeled CpG-C 24 hours before sacrifice, and single cells suspensions were stained using anti-CD31 (PCAM-1) PE-Cy7 (eBioscience), Anti-GLAST (ACSA-1)-PE (MACS), and anti-CD11b APC (BioGems).

Techniques: Labeling, Injection, Confocal Microscopy